ISSN: 1859 - 2872
Evaluating the value of RT-PCR in identifying Gram positive bacteria caused sepsis
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Abstract
Objective: To evaluate a in-house Realtime PCR (RT-PCR) assay in identifying Gram positive bacteria causing sepsis. Subject and method: 170 sepsis patients accordding to Sepsip-3 were recruited into this study. Causative pathogens were identified by conventional blood culture (BC) and RT-PCR concomitantly. Result: 34.1%, 55.3% and 59.4% patients were identified bacterial pathogens in blood samples by BC, RT-PCR, and combination of two assays, respectively. Among those positive patients with RT-PCR, 27.7% (26/94) cases were positive for Gram positive bacteria. 43 out of 112 cases negative with BC were positive with RT-PCR and the proportion of Gram positive bacteria in those were 39.5% (17/43). RT-PCR identified pathogens significantly higher than BC (72.4% vs 10.3%, p<0.05), especially several bacteria like Streptococcus sp and Enterococcus sp were only detected by RT-PCR. Conclusion: RT-PCR assay was an effective assay in identifying Gram positive bacteria in sepsis patients.
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References
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