The value of real-time PCR in detecting anaerobes in blood of patients with sepsis

  • Nguyễn Sỹ Thấu Học viện Quân y
  • Ngô Tất Trung Bệnh viện Trung ương Quân đội 108
  • Vương Phúc Đường Bệnh viện Trung ương Quân đội 108
  • Lê Hữu Song Bệnh viện Trung ương Quân đội 108

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Keywords

real-time PCR, Sepsis

Abstract

Objective: To evaluate the benefits of real-time PCR (RT-PCR) method to detect anaerobes in blood of patients with sepsis. Subject and method: 92 sepsis patients were enrolled on this study. Pathogen identification in each patient was performed by using both blood culture and RT-PCR concomitantly. Result: The rate of positive of culture, RT-PCR, combine culture and RT-PCR is 34.78%, 51.09%, 56.52% respectively. In total of 60 cases negative with blood culture there are 20 cases were positive with RT-PCR. In contrast, 5 cases were positive by blood culture but negative by RT-PCR. RT-PCR has been shown more advantage than blood culture since it can detect some bacteria S. aureus, S. pneumoniae, Enterococcus sp, especially obligate anaerobes like Bacteroides sp which were not detected by blood culture at the same patients. Conclusion: It has been shown that RT-PCR is one of potential support assay for detecting anaerobes in blood of patients with sepsis.

Article Details

References

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Published
Feb 12, 2018
Issue
Volume 13 - No 1/2018
Section
Articles
How to Cite
ThấuN. S., TrungN. T., ĐườngV. P., & SongL. H. (2018). The value of real-time PCR in detecting anaerobes in blood of patients with sepsis. Tạp Chí Y Dược lâm sàng 108, 13(1). Retrieved from https://tcydls108.benhvien108.vn/index.php/YDLS/article/view/242