Characteristic of pathogen and effectiveness of diagnosis between blood culture and real-time PCR in septic patients

  • Lien Tran Thi Bệnh viện Hữu nghị Việt Tiệp
  • Dong Do Van Bệnh viện Trung ương Quân đội 108
  • Sang Vu Viet Bệnh viện Trung ương Quân đội 108
  • Trung Ngo Tat Bệnh viện Trung ương Quân đội 108

Main Article Content

Keywords

Sepsis, blood culture, real-time PCR

Abstract

Objective: To find out pathogens and evaluate the effectiveness of diagnosis between blood culture and real-time PCR in septic patients. Subject and method: 105 septic patients according to Sepsip-3 were recruited into this study. Causative pathogens were identified by conventional blood culture (BC) and/or RT-PCR concomitant. Result: By blood culture method, 74 pathogens were identified, E. coli (14.3%), K. pneumoniae (10.5%) and S. aureus (12.4%) were the most common bacteria. Among 76 septic patients who were conducted 2 methods, real-time PCR detected 50 pathogens and E. coli (19.7%), K. pneumoniae (13.2%) were the most common bacteria. The rate of positive real-time PCR was higher than that of blood culture (65.8% vs 53.9%, p<0.05). Combining 2 methods gave a positive of 84.2%. The rate of positive similarity between the two methods was 81.5% (22/27). Conclusion: E. coli, K. pneumoniae and S. aureus were the most common pathogens caused sepsis and real-time PCR assay was an effective assay in identifying pathogens in sepsis patients

Article Details

References

1. CDC Data & Report, https://www.cdc.gov/sepsis/ datareports/index.html.
2. Sterling SA, Miller WR, Pryor J et al (2015) The impact of timing of antibiotics on outcomes in severe sepsis and septic shock: A systematic review and meta-analysis. Critical care medicine 43(9): 1907-1915.
3. Suberviola B, Márquez-López A, Castellanos-Ortega A et al (2016) Microbiological diagnosis of sepsis: Polymerase chain reaction system versus blood cultures. American Journal of Critical Care 25(1): 68-75.
4. V. Q. Dat, H. N. Vu, H. Nguyen The et al (2017) Bacterial bloodstream infections in a tertiary infectious diseases hospital in Northern Vietnam: Aetiology, drug resistance, and treatment outcome. BMC infectious diseases 17(1): 493-493.
5. Uslan DZ, Crane SJ, Steckelberg JM et al (2007) Age and sex-associated trends in bloodstream infection: A population-based study in olmsted county, minnesota. JAMA Internal Medicine 167(8): 834-839.
6. Tsalik EL, Jones D, Nicholson B et al (2010) Multiplex PCR to diagnose bloodstream infections in patients admitted from the Emergency Department with sepsis. Journal of Clinical Microbiology 48(1): 26-33.
7. Scheer CS, Fuchs C, Gründling M et al (2019) Impact of antibiotic administration on blood culture positivity at the beginning of sepsis: A prospective clinical cohort study. Clinical Microbiology and Infection 25(3): 326-331.
8. IŞÇimen R, Girgin NK and Kahveci F (2016) Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis. Current Contents, SciSearch, PubMed/MEDLINE, EMBASE, Scopus 350(3): 301-309.
9. Rangel-Frausto MS, Pittet D, Costigan M et al (1995) The natural history of the systemic inflammatory response syndrome (SIRS): A prospective Study. JAMA 273(2): 117-123.

Article Sidebar

PDF (Tiếng Việt)
Published
Aug 27, 2019
Issue
Volume 14 - No 6/2019
Section
Articles
How to Cite
Tran ThiL., Do VanD., Vu VietS., & Ngo TatT. (2019). Characteristic of pathogen and effectiveness of diagnosis between blood culture and real-time PCR in septic patients. Tạp Chí Y Dược lâm sàng 108, 14(6). Retrieved from https://tcydls108.benhvien108.vn/index.php/YDLS/article/view/117