ISSN: 1859 - 2872
Evaluation of mobilized peripheral blood bone marrow stem cells from patients with decompensated cirrhosis due to hepatitis B virus
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Abstract
Objective: Collecting mononuclear cell (MNC) containing CD34+ cells in peripheral blood from patients with HBV-related compensation liver cirhosis after G-CSF imbomilization and evaluating the quality of bone marrow stem cells in MNC product. Subject and method: 31 patients with decompensated cirrhosis due to hepatitis B virus (ages 35 - 78) were enrolled in the study from June 2016 to August 2019. 60MIU neupogen was use twice daily subcutaneous injection in 3-5 days. CD34 plus cells were collected when peripheral CD34+ reaching at least 10 cells/µl. The quality of the product was determined via the number and viability of CD34+ cells. Result: The duration for mobilizaion was 4.35 (3 - 6 days). The number of peripheral CD34+ was 22.0 cells/µl (10.16 - 76.5). The cutoff value of peripheral blood CD34+ at 15 cells/µl possibly predicts to achieve expecting treatment dose as 106 cells/kg. The CD34+/kg dose was 1.59x106 cells/kg. Conclusion: 4-day mobilization using subcutaneous 60MIU Neupogen was efficacy and safety for collecting CD34+ from peripheral blood
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References
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