ISSN: 1859 - 2872
Rate of formation and quality of blastocyst in embryo culture with low oxygen levels
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Abstract
Objective: To compare rates of formation and quality of embryos. Subject and method: The patients were using FSH to stimulate ovaries. Oocytes retrieval after hCG injection 36 hours. Fertilization by conventional IVF or ICSI. In both groups, in day 2, 3 - 6 embryos were selected to culture in day 3. Patients with ≥ 3 good quality embryos in day 3 will be grown in the next 5th day. Result: There was not differences of characteristic, ovarian reserve, ovarian stimulation and embryos transfer in both groups. Embryo formation rate (%) in 5% oxygen concentration group was 90.04% ± 17.86% significantly higher than 74.44% ± 26.63% in 20% oxygen concentration group (p=0.0001). The percentage of good quality embryos cultured in 5% oxygen concentration group was 79.90% ± 26.04% higher than 73.61% ± 35.30% in 20% oxygen concentration group (p=0.02). Conclusion: The rate of formation embryos cultured in 5% oxygen concentration group was significantly higher than in 20% oxygen group.
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References
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