ISSN: 1859 - 2872
Formulation of phytosomes containing bioactive constituents from Herba cistanches
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Abstract
Objective: To formulate and characterize phytosome incorporating bioactive constituents extracted from Herba Cistanches (phytosome NTD). Subject and method: The preparation of Phytosome NTD was achieved through a combination of solvent evaporation and lyophilization techniques. The study employed entrapment efficiency, particle size, and polydispersity index (PDI) to examine the influence of various factors, including ethanol concentration, solvent volume, NTD extract/lecithin ratio, reaction temperature, and reaction time. Furthermore, assessments were conducted on morphology, particle size, PDI, zeta potential, FT-IR spectroscopy, differential scanning calorimetry, and the oil–water partition coefficient. Result: Under ultrasound 37 kHz conditions, phytosome NTD was obtained with the following conditions: A ratio of NTD extract/lecithin of 1/1; a reaction solvent of 40% EtOH; a solid/solvent ratio of 1/50; a reaction temperature of 70°C; and a reaction time of 4 hours. Phytosome is spherical in shape, with particle size < 200 nm, polydispersity index < 0.3, and a zeta potential of around -25.77mV, entrapment efficiency of about 75%. Conclusion: Phytosome NTD was successfully formulated, and several of its important physicochemical characteristics were evaluated.
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References
2. Gao Y, Zong C, Liu F et al (2015) Evaluation of the intestinal transport of a phenylethanoid glycoside-rich extract from Cistanche deserticola across the Caco-2 cell monolayer model. PloS one, 10(2):e0116490.
3. Jiang Z, Wang J, Li X et al (2016) Echinacoside and Cistanche tubulosa (Schenk) R. wight ameliorate bisphenol A-induced testicular and sperm damage in rats through gonad axis regulated steroidogenic enzymes. Journal of Ethnopharmacology 193: 321-328.
4. Wang W, Jiang S, Zhao Y et al (2023) Echinacoside: A promising active natural products and pharmacological agents. Pharmacological Research 197(106951).
5. Dutt Y, Pandey RP, Dutt M et al (2023) Liposomes and phytosomes: Nanocarrier systems and their applications for the delivery of phytoconstituents. Coordination Chemistry Reviews 491: 215-251
6. Dược điển Việt Nam, Nhục thung dung
7. Rene P Schwarzenbach, Philip M Gschwend, Dieter M Imboden (2016) Environmental Organic Chemistry, 3rd Edition. Wiley, ISBN: 978-1-118-76723-8, 298-300.
8. Fei Y, Yang L, Qin C et al (2016) Monodisperse microparticles loaded with the self-assembled berberine-phospholipid complex-based phytosomes for improving oral bioavailability and enhancing hypoglycemic efficiency. European Journal of Pharmaceutics and Biopharmaceutics, 103 (136-148.
9. Li F, Yang X, Yang Y et al (2015) Phospholipid complex as an approach for bioavailability enhancement of echinacoside. Drug Development and Industrial Pharmacy, 41(11): 1777-1784.
10. Han SY, Qiao JQ, Zhang YY et al (2012) Determination of n-octanol/water partition coefficients of weak ionizable solutes by RP-HPLC with neutral model compounds. Talanta 97: 355-361.