Studying some hippocampal characteristics and the results of 0.9% NaCl injection in the hippocampus in rat
Main Article Content
Keywords
Abstract
Objective: To determine some characteristics of hippocampal size and hippocampal injection coordinates and comment on hippocampal injection results in rat. Subject and method: A descriptive controlled experimental study of 18 healthy adult rats weighing 210-240g. Result: Distance of needle to hippocampal inner margin (1.71mm), outer margin (1.56mm), dorsal (1.22mm), ventral (1.38mm), head (2.21mm) and tail hippocampus (2.3mm); Distance from dorsal hippocampus to parietal cortex was 2.11mm. Conclusion: The hippocampal measures are antero-posterior of 4.26mm, medio-lateral of 3.15mm and dorso-ventral of 2.49mm. The coordinates inject into CA1 region of hippocampus is AP = - (4.4 ± 0.8) mm; ML = ± (2.2 ± 0.2) mm; DV = - (3.1 ± 0.1) mm. Injecting 2.5μl of solution into the hippocampus have not seen affect on the rat health.
Article Details
References
2. Facchinetti R, Bronzuoli MR, & Scuderi C (2018) An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid β-Peptide (1-42). Methods in molecular biology (Clifton, N.J.) 1727: 343-352.
3. Arifin WN, Zahiruddin WM (2017) Sample size calculation in animal studies using resource equation approach. Malays J Med Sci 24(5): 101-105.
4. Wang DL, Ling ZQ, Cao FY et al (2004) Melatonin attenuates isoproterenol-induced protein kinase A overactivation and tau hyperphosphorylation in rat brain. J Pineal Res 37(1): 11-16.
5. Kjonigsen LJ, Leergaard TB, Witter MP et al (2011) Digital atlas of anatomical subdivisions and boundaries of the rat hippocampal region. Frontiers in neuroinformatics 5: 2.
6. Chauhan P, Jethwa K, Rathawa A et al (2021) The Anatomy of the Hippocampus. Cerebral Ischemia.
7. Tatu L, Vuillier F (2014) Structure and vascularization of the human hippocampus. Front Neurol Neurosci 34: 18-25.
8. Rao YL, Ganaraja B, Murlimanju BV et al (2022) Hippocampus and its involvement in Alzheimer's disease: A review. Biotech 12(2):55.
9. Yang P, Wang Z, Zhang Z et al (2018) The extended application of The Rat Brain in Stereotaxic Coordinates in rats of various body weight. J Neurosci Methods 307: 60-69.
10. Franklin & Paxinos (2007) The Mouse Brain in Stereotaxic Coordinates. Elsevier, 3rd Edition.