ISSN: 1859 - 2872
Identification of bacteria and yeast by Maldi-Tof Vitek MS and Titek 2 Compact
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Abstract
Objective: To compare the identification of bacteria and yeast by Maldi-Tof Vitek and Vitek 2 compact systems. Subject and method: 260 colonies isolated from clinical samples at 108 Military Central Hospital from September, 2019 to October, 2019 were identified by using two Maldi-Top Vitek MS and Vitek 2 systems. Result: 260 isolates were successfully identified by both methos, include 241 bacteria strains and 19 yeast strains belonging into 40 genus. Common bacteria strains isolated E. coli, K. pneumoniae, Acinetobacter… Of those, 253 strains were (97.31%) completely match in identification by both methods in genus level and 7.31% trains were differently identified in species level. We found that 7 isolates (2.69%) were inaccordantly identified by both methods in genus level. The average time for identification of negative bacteria, positive bacteria, yeast by Vitek 2 compact systems were 306, 308, and 1079 minutes, respectively. Meanwhile Maldi-Tof Vitek MS showed identification after several minutes for bacteria as well as yeast. Conclusion: Maldi-Tof Vitek MS rapidly and accurately identifies bacteria and yeast. The limitation of this study is lack of the 16S sequencing method as the gold standard method for comfirming bacteria and yeast strains which are unmatch in identification by Maldi-Tof Vitek MS and Vitek 2 compact systems.
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References
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