Tác nhân gây bệnh trong viêm mủ nội nhãn nội sinh do vi khuẩn

Abstract

Objective: To characterize the pathogens of bacterial endogenous endophthalmitis (BEE). Subject and method: Analysing vitreous samples with cellular and molecular techniques from 110 BEE patients who had been treated with vitrectomy at Vietnam National Institute of Ophthalmology over 2 years of 2012 and 2013. Result: Positive rate was low of 10.9% with culture and the inconsistancy did exist between Gram staining and culture. PCR and sequencing got better sensitivity of 54% with Gram (+) accounting for 54.2% (32/59) of PCR detected cases. Gram (-) rods (P. aeruginosa, K. pneumoniae, Stenotrophomonas sp., Enterobacter and P. maltophilia) were rarely seen accounting for 1.7 - 10.2%. Conclusion: Although culture remained gold standard for diagnosis and treatment of infectious diseases, it had limited use if BEE due to low positive rate. Biological diagnosis in BEE should base on the combination of Gram staining, culture and PCR-sequencing.