ISSN: 1859 - 2872
Research on improving memory, cognition and anti-inflammatory of adipose tissue-derived mesenchymal stem cells and exosomes on experimental Alzheimer's disease model
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Abstract
Objective: To evaluate some behavioral symptoms, hippocampal biochemistry in Alzheimer's disease (AD) modeling rats after treatment with human adipose tissue-derived mesenchymal stem cells (hADSC) and exosome (EX). Subject and method: 24 rats were modeled AD, longitudinal descriptive study, AD modeling rats treated with hADSC and EX. Testing and analyzing behavior on the Morris water maze and object recognition task. Biochemical assay to quantify inflammatory cytokines in the hippocampus. Result: The latency time on the 5th day of learning on the water maze of AD model rats after treatment with EX, hADSC were (9.03 ± 1.61 s), (8.23 ± 1.10 s), respectively. The number of times rats crossed the platform in the probe test in the water maze of the EX treatment group was (3.25 ± 0.37), hADSC was (3.50 ± 0.60). The object recognition index in the open field of the EX treatment group was (84.05 ± 7.67%), hADSC was (83.50 ± 5.08%). Inflammatory cytokine levels in the hippocampus after treatment with EX were TNFα (26.21 ± 0.95 pg/mg), IL1β (221.29 ± 12.72 pg/mg). After treatment with hADSC were TNFα (25.61 ± 1.64 pg/mg), IL1β (237.03 ± 10.95 pg/mg). Conclusion: Adipose-derived mesenchymal stem cells, exosomes improve learning, memory and reduce neuroinflammation in the hippocampus in Alzheimer's disease modeling rats.
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References
2. Facchinetti R, Bronzuoli M, Scuderi C (2018) An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid β-Peptide (1-42). Methods in molecular biology (Clifton, N.J.) (1727): 343-352.
3. Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8(4): 315-317.
4. Toyserkani NM, Christensen ML, Sheikh SP et al (2014) Adipose-derived stem cells: new treatment for wound healing? Annals of Plastic Surgery 75(1): 117.
5. Rani S, Ryan AE, Griffin MD, et al (2015) Mesenchymal stem cellderived extracellular vesicles: toward cell-free therapeutic applications. Molecular Therapy 23(5): 812-823.
6. Lueptow LM (2017) Novel Object Recognition Test for the Investigation of Learning and Memory in Mice. Journal of visualized experiments: JoVE, (126): 55718.
7. Vorhees CV, & Williams MT (2006) Morris water maze: procedures for assessing spatial and related forms of learning and memory. Nature protocols 1(2): 848-858.
8. Choi JM, Park HS, He MT et al (2022) Membrane-free stem cells and pyridoxal 5'-phosphate synergistically enhance cognitive function in Alzheimer's disease mouse model. Antioxidants (Basel, Switzerland) 11(3): 601.
9. Mehrabadi S, Motevaseli E, Sadr S et al (2020) Hypoxic-conditioned medium from adipose tissue mesenchymal stem cells improved neuroinflammation through alternation of toll like receptor (TLR) 2 and TLR4 expression in model of Alzheimer's disease rats. Behavioural brain research 379: 112362.
10. Othman MZ, Hassan Z Che Has AT (2022) Morris water maze: a versatile and pertinent tool for assessing spatial learning and memory. Exp Anim, 71(3): 264-280.
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