Nghiên cứu đặc điểm kiểu hình miễn dịch ở bệnh nhân lơ xê mi cấp tại Bệnh viện Trung ương Quân đội 108

Abstract

Objective: To describe the features of immunophenotyping in acute leukemia patients who were newly diagnosed in the Department of Hematology, 108 Military Central Hospital from 2016 to 2020. Subject and method: 155 patients were diagnosed as acute leukemia (101 male, 54 female), each patient was aspirated about 0.5 - 1.5ml of bone marrow fluid and peripheral blood to perform complete blood count and myelogram and immunophenotyings were perform by 4 colors FACS Calibur flow cytometry analysis system of Becton Dickinson (USA). Result: The proportions of patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) were 74.19% and 25.81%, respectively. In AML patients, myeloblast population cells were positive with CD33, CD117, CD13 and MPO; monoblast population was positive with CD4, CD14, and CD64; erythroblast population was positive with CD71, Glycophorin A; megakaryoblast was positive for CD41. ALL-B patients were positive for CD19, Cy79a and some were positive for CD10; ALL-T patients were positive for CD3, CD4, CD5, CD7 and CyCD3. Conclusion: Characteristics of immunophenotyping in acute leukemia in patients at 108 Military Central Hospital from January 2016 to November 2020 was mainly myeloid linage leukemia, in addition, it could also be classified to specifically cell linage and there was both B and T cell linage leukemia.