ISSN: 1859 - 2872
Evaluation of in-house internal quality control samples for serum indices
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Abstract
Objective: To identify the homogeneity and stability of in-house internal quality control samples (IQC) for serum indices at Biochemistry Department, 108 Military Central Hospital. Subject and method: A 1200ml normal plasma sample pool that was clear, light yellow, having serum indices values: < 0.25 index for hemolysis, < 30.0 index for icteria, < 0.3 index for lipemia, respectively and 3 types of plasma samples: 150ml plasma with artificial hemolysis, 150ml plasma with over 360μmol/L total bilirubin concentration, 150ml plasma adding 20% intralipid up to triglyceride concentration of 20mmol/l. The IQC samples for HIL indices were prepared by mixing these previous plasma samples with normal plasma sample pool at 1: 1 ratio, including 3 levels: High, medium, low concentrations. Evaluation of short-term (20 days) and long-term (90 days) stability of the IQC samples was based on CLSI EP05-A2 guidelines. Result: For the homogeneity: There was no difference of HIL values of all IQC samples between 2 analysis for each concentration, p>0.05. The IQC samples were stable at 4oC for 20 days (CVr 0.2 - 4.15, CVt 0.18 - 4.64), at 4oC for 90 days (CVr 0.19 - 4.51, CVt 0.17 - 4.72), at -20oC for 20 days (CVr 0.15 - 2.96, CVt 0.14 - 3.14), and at -20oC for 90 days (CVr 0.14 - 2.79, CVt 0.14 - 3.23). Conclusion: The in-house IQC for HIL indices ensured the homogeneity, short-term stability (20 days) and long-term stability (90 days) at 4oC and -20oC storage.
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References
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