ISSN: 1859 - 2872
Colistin resistance in multidrug and carbapenem resistant Klebsiella pneumoniae isolated in the 108 Military Central Hospital from 1/2020 to 9/2020
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Abstract
Objective: To determine the prevalence of colistin resistance in multidrug and carbapenem resistant Klebsiella pneumoniae isolated at the 108 Military Central Hospital during the period from 01/2020 to 09/2020. Subject and method: A total of 86 K. pneumoniae strains were isolated from different clinical specimens. Bacteria were identified and antibiotic susceptibility testing was performed using Vitek-2 compact system. Multidrug/Carbapenems-resistant K. pneumoniae strains were subjected to colistin susceptibility testing using disc diffusion method on Muller-Hinton medium with colistin broth disk elution (CBDE) and the minimum inhibitory concentration (MIC) was determined accordingly. Result: Prevalence of multidrug/carbapenem-resistant K. pneumoniae (MD/CRKP) was 20.05% (86/429). The rate of MD/CRKP was the highest in Intensive Care Unit (35.0%, 30/86), followed by Infection Institute (17.0%, 15/86) and Department of Gastroenterology (8.0%, 7/86). MD/CRKP strains were mainly recovered from respiratory 55.81% (48/86) and 20.93% (18/86) blood specimens, 12.79% (11/86) urine speciment, 10.47% (9/86) fluit speciment. 45.0% (39/86) of MD/CRKP were resistant to colistin ((MIC ≥ 4mg/mL) and 15.0% (13/86) others displayed also MIC colistin increased (1mg/mL < MIC ≤ 2mg/mL). Conclusion: MD/CRKP resistant to colistin has emereged in the clinical setting, therefore, it is necessary to implement a practical guide for ensuring appropriate antimicrobial use in the hospital as well as to introduce diagnostic tools for rapid detection of colistin resistant strains.
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References
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