Nghiên cứu một số đặc điểm và tác động của tế bào gốc phân lập được từ mô mỡ lên nguyên bào sợi da trên in vitro

Abstract

Objective: To describe some charateristics of Adipose-Derived Stem Cells (ADSCs) and to assess the effects of adipose-derived stem cells (ADSCs) on dermal fibroblasts responses via migration and proliferation in vitro. Subject and method: 10 samples of dermal fibroblasts and ADSCs were isolated from skin and adipose tissues of 10 healthy persons, who hospitalized at Plastic surgery Department, National Burn Hospital for plastic surgeries from June to December, 2020. We counted ADSCs after isolation and determined the morphological characterisation of ADSCs afer 72 hours, 7 days, 10 days of culture and chromosomes characterisation of ADSCs at the 5^th passage. We also determined the effects of ADSCs on the proliferation and migration of dermal fibroblasts in vitro. Result: The average number of ADSCs after isolation was 2.63×10⁶ ± 1.6×10⁶ cells/1 gram of adipose tissue (min - max: 2.5 - 2.7×10⁶) and the percentage of healthy ADSCS was 96.6 ± 1.5% (min - max: 96.2 - 97.5). After 72 hours of culture, ADSCs adhered to the surface of cell culture plate. After 7 days of culture, ADSCs spreading was promoted by coating the cell culture plate. After 10 days of culture, the proliferation of ADSCs occurred strongly, they coved more than 80% of the surface area of cell culture plate. The ADSCs still had normal chromosomal structure in the 5^th passage. The proliferation and migration rate of dermal fibroblasts in the culture medium which supplemented with ADSCs -conditionned medium (ADSCs-CM) was higher than that in the control group. These rates were the highest when replacing 50% of the culture medium of dermal fibroblasts with ADSCs-CM. Conclusion: The ADSCS that we isolated had all the morphological characterisation of the stem cells and still retained the normal chromosomal structure in the 5^th passage. In vitro, ADSCs stimulated the proliferation and migration of dermal fibroblasts.