ISSN: 1859 - 2872
Molecular characterization of Fasciola isolates in Vietnam based on ITS1 sequence of ribosomal DNA
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Abstract
Objective: The aim of the present study was to examine nucleotide variation in the first Internal Transcribed Spacers (ITS-1) region of nuclear ribosomal DNA genes of Fasciola in Vietnam. Subject and method: Sixteen adult Fasciola spp. were collected from buffalo (5 isolates), bovine (6 isolates), sheep (2 isolates), goats (2 isolates), and human (1 isolate) from slaughterhouse in Hanoi city (4 isolates), Vinh Phuc (1 isolate), Nghe An (2 isolates), Quang Nam (1 isolate), Ninh Thuan (4 isolates), Tay Ninh province (1 isolate), Can Tho city (1 isolate), and Dong Thap province (2 isolates), Vietnam, from 2009 to 2016. The ribosomal ITS1 and mitochondrial genes of nad1 from individual Fasciola isolates were amplified and the PCR products were sequenced by both directions. Nucleotide variation within and between the individuals was evaluated by comparing the sequences of ribosomal ITS1 region. Result: Based on nad1 and ITS1 sequences, 11 isolates were identified as F. gigantica and 5 isolates were identified as intermediate form (Fasciola sp.). Based on ITS1 sequence, five DNA variable sites were observed in which single-base substitution at different sites was occurred and four haplotypes were present in Vietnam. Conclusion: Findings of the study showed that high genetic variation on ITS1 marker was noted in Vietnam isolates in comparison with isolates from other geographical regions of the world.
Keywords: Nucleotide variation, ITS1 region, Fasciola, Vietnam.
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References
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