ISSN: 1859 - 2872
Developing and applying novel assay for detecting MTHFR polymorphisms for women with recurrent pregnancy loss
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Abstract
Objective: To develop a fast and accurate testing method for polymorphisms at 2 points of MTHFR gene (677 and 1298) by realtime PCR method and initially evaluate the value of the genotype polymorphism test in diagnosis, predict and guide treatment for recurrent pregnancy loss. Subject and method: Descriptive study of vertical track. The realtime PCR assay method is formulated and optimized, then evaluated against using the commercial kit CVD StripAssay® T (ViennaLab). 200 samples were collected from normal people to determine the genotype rate of the population and 48 samples from patients with a history of recurrent pregnancy loss were used to evaluate the effect of polymorphism of MTHFR gene at 2 points 677 and 1298. Result and conclusion: Realtime PCR method was developed capable of accurately identifying polymorphisms of the MTHFR gene with low sample DNA concentration (2 - 5ng/µL) with the advantage of a fast and limited interference signal. The study also showed the MTHFR genotype model with 2 mutation points 677 and 1298 in the Vietnamese population. It also shows a significant correlation between the high rate of heterozygous genotypes with women have recurrent pregnancy loss (RPL). This proposes that MTHFR genotype identification is a potential marker to identify the cause of female infertility, predict the state of miscarriage as well as propose appropriate treatment.
Keywords: Recurrent pregnancy loss, MTHFR polymorphisms, genotype rate.
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References
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