ISSN: 1859 - 2872
Preliminary investigation of stability of some biochemical tests in whole blood and plasma in storage time conditions
Main Article Content
Keywords
Abstract
Objective: To assess effects of storage time condition on results of urea, creatinine, triglyceride, cholesterol, HDL-cholesterol, LDL-cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyltransferase (GGT), acid uric, total bilirubin, direct bilirubin in whole blood and plasma samples. Subject and method: 162 whole blood samples of 81 patients examined at Department of Examination - 108 Military Central Hospital from 15/11/2020 to 15/02/2021 that were collected in lithium heparin tubes (2 tubes per patient). After each patient was collected whole blood, the first tube was immediately centrifuged and analyzed routinely, the remaining sample was labelled ‘sample 1’, the second tube was separated plasma from blood cells (labelled ‘sample 2’). Then both samples were kept in refrigerator at 4oC. All of these samples were analyzed biochemical tests in 24, 48, 72 hours after blood collection. Result: The concentrations of AST of samples 1 stored in 24, 48, 72 hours were higher than the AST levels of routine analysis samples (p<0.05). There was a decrease in the concentrations of total bilirubin and direct bilirubin of both samples 1 and samples 2 during their storage time (p<0.05). Conclusion: The concentrations of AST, total bilirubin and direct bilirubin of stored samples (plasma and whole blood samples) were unstable at 4oC during their storage time. The concentrations of urea, creatinine, triglycerid, cholesterol, HDL-cholesterol, LDL-cholesterol, ALT, GGT, acid uric (of both plasma and whole blood samples) showed stability up to 72 hours at 4oC.
Keywords: Clinical chemistry, specimen storage, 108 Military Central Hospital.
Article Details
References
2. Lippi G et al (2006) Influence of hemolysis on routine clinical chemistry testing. Clin Chem Lab Med 44(3)(1434-6621): 311-316.
3. Okada H et al (2012) Influence of bilirubin photoisomers on unbound bilirubin measurement in clinical settings. Ann Clin Biochem 49(6)(1758-1001 (Electronic)): 595-599.
4. Oddoze C, H Lombard E Fau - Portugal, and Portugal H (2012) Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin Biochem 45(6)(1873-2933 (Electronic)): 464-469.
5. Flores CFY et al (2020) Sample management: stability of plasma and serum on different storage conditions. EJIFCC 31(1): 46-55.
6. Boyanton BL, and Blick KE (2002) Stability studies of twenty-four analytes in human plasma and serum. Clin Chem 48(12)(0009-9147 (Print)): 2242-2247.
7. Tambse V (2015) Study of the Stability of various Biochemical Analytes in Pooled Sera preserved at 4 - 8oC. Asian Journal of Biomedical and Pharmaceutical Sciences 05(48): 38-39.
8. Heins M, Heil W, Fau - Withold W, and Withold W
(1995) Storage of serum or whole blood samples? Effects of time and temperature on 22 serum analytes. Eur J Clin Chem Clin Biochem 33(4)(0939-4974 (Print)): 231-238.
9. Pawlik-Sobecka L et al (2020) The influence of serum sample storage conditions on selected laboratory parameters related to oxidative stress: A preliminary study. Diagnostics (Basel, Switzerland) 10(1): 51.
10. O'Keane MP and Cunningham SK (2006) Evaluation of three different specimen types (serum, plasma lithium heparin and serum gel separator) for analysis of certain analytes: Clinical significance of differences in results and efficiency in use. Clin Chem Lab Med 44(5)(1434-6621 (Print)): 662-668.
Article Sidebar
