Establishing a novel realtime RT-PCR to detect Zika and Chikungunya viruses

  • Đào Thanh Quyên Bệnh viện Trung ương Quân đội 108
  • Ngô Tất Trung Bệnh viện Trung ương Quân đội 108
  • Bùi Tiến Sỹ Bệnh viện Trung ương Quân đội 108
  • Trần Thị Huyền Trang Bệnh viện Trung ương Quân đội 108
  • Đào Phương Giang Bệnh viện Trung ương Quân đội 108
  • Trần Thị Thu Hiền Bệnh viện Trung ương Quân đội 108
  • Nguyễn Thị Tĩnh Bệnh viện Trung ương Quân đội 108
  • Trần Thái Hà Bệnh viện Trung ương Quân đội 108
  • Nguyễn Việt Hà Bệnh viện Trung ương Quân đội 108
  • Trần Đức Anh Bệnh viện Trung ương Quân đội 108
  • Hồ Thị Hường Bệnh viện Trung ương Quân đội 108
  • Phan Quốc Hoàn Bệnh viện Trung ương Quân đội 108

Main Article Content

Keywords

Realtime RT-PCR, Zika virus, Chikungunya virus

Abstract

Objective: To establish of a real-time RT-PCR method for the detection of Zika and Chikungunia viruses. Subject and method: This real-time RT-PCR-based method is for the amplification of target genes, using relevant negative, ZIKV and CHIKV controls that were provided by NIHE and WHO, respectively. Detection limits and sensitivity were determined by a range of spiking assays from 105÷100 copies/µl. Result: Our data demonstrated that the LOD of our newly developed method for detecting Zika virus and Chikungunia virus were 5 and 3 RNA copies/µl that are equal to 500 and 300 copies/ml. Specificity and sensitivity were 100 - 95%, respectively. Conclusion: A real-time RT-PCR assay for the detection of Zika virus and Chikungunia virus was succesfully established.

Article Details

References

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Published
Mar 31, 2021
DOI: https://doi.org/10.52389/ydls.v16i1.693
Issue
Volume 16 - No 1/2021
Section
Articles
How to Cite
Quyên Đào T., TrungN. T., SỹB. T., TrangT. T. H., Giang Đào P., HiềnT. T. T., TĩnhN. T., HàT. T., HàN. V., AnhT. Đức, HườngH. T., & HoànP. Q. (2021). Establishing a novel realtime RT-PCR to detect Zika and Chikungunya viruses. Tạp Chí Y Dược lâm sàng 108, 16(1). https://doi.org/10.52389/ydls.v16i1.693