ISSN: 1859 - 2872
Development of an effective solvent for total RNA extraction from urine specimen for detecting microbiological pathogens
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Keywords
RNA extraction, 108@Uzol, Taqman-probe
Abstract
Objective: To successfully prepare solvents to extraction total RNA from urine specimens. Subject and method: Using existing chemicals that are readily available, blended into solvents to extract total RNA from urine specimens. Quantitative by real time PCR technique according to Taqman Probe principle to evaluate the efficiency of RNA separation with self-created solvent. Result: Successfully prepared solvent to separate total RNA from urine with extraction efficiency equivalent to that of commercial counterparts. Conclusion: Solvents for extraction of total RNA from urine were successfully prepared with extraction efficiency for detecting microbiological pathogens.
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References
1. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162(1): 156-159.
2. Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat Protoc 1(2): 581-585.
3. Hamel R, Dejarnac O, Wichit S, Ekchariyawat P, Neyret A, Luplertlop N, Perera-Lecoin M, Surasombatpattana P, Talignani L, Thomas F, Cao-Lormeau VM, Choumet V, Briant L, Desprès P, Amara A, Yssel H, Missé D (2015) Biology of Zika virus infection in human skin cells. J Virol 89(17): 8880-8896.
4. Lyle R, Petersen DJJ, Ann M Powers, and Margaret A Honein (2016) Zika virus. N Engl J Med 374: 1552-1563.
2. Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat Protoc 1(2): 581-585.
3. Hamel R, Dejarnac O, Wichit S, Ekchariyawat P, Neyret A, Luplertlop N, Perera-Lecoin M, Surasombatpattana P, Talignani L, Thomas F, Cao-Lormeau VM, Choumet V, Briant L, Desprès P, Amara A, Yssel H, Missé D (2015) Biology of Zika virus infection in human skin cells. J Virol 89(17): 8880-8896.
4. Lyle R, Petersen DJJ, Ann M Powers, and Margaret A Honein (2016) Zika virus. N Engl J Med 374: 1552-1563.
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Published
Mar 31, 2021
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Articles
How to Cite
Giang Đào P., TrungN. T., HiềnT. T. T., TĩnhN. T., & HoànP. Q. (2021). Development of an effective solvent for total RNA extraction from urine specimen for detecting microbiological pathogens. Tạp Chí Y Dược lâm sàng 108, 16(1). https://doi.org/10.52389/ydls.v16i1.691