ISSN: 1859 - 2872
Determination of Fasciola species isolated from bovine and buffalo in the Northern Vietnam using PCR-RFLP method
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Abstract
Summary
Objective: The present study was aimed to identify the genotype of Fasciola spp. isolated from bovine and buffalo in the Northern Vietnam using PCR-RFLP method based on rDNA (ITS1) markers. Subject and method: Adult Fasciola spp. were isolated from bile ducts of bovine and buffalo in four province in the Northern Vietnam. Overall, 122 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 65 isolates from infected bovine, 57 isolates from infected buffalo. 122/122 isolates were identified as Fasciola species by ITS1 of rDNA. In this study, PCR-RFLP methods was used to distinguish between F. hepatica and F. gigantica according to the previous study. Result: The present results showed that, using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the rDNA revealed that 95 out of 122 isolates (77.9%) were Fasciola gigantica type, whereas 27 isolates (22.1%) presented an intermediate Fasciola. No F. hepatica was detected in the present study. The both types of Fasciola were determined in four province and in bovine and buffalo in the Northern Vietnam. Conclusion: F. gigantica and intermediate form were identified in bovine and buffalo in the Northern Vietnam, whereas F. hepatica was not detected.
Keywords: Fasciola, bovine, buffalo, PCR-RFLP, Northern Vietnam.
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References
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