ISSN: 1859 - 2872
The value of genotype in identifying carbapenem resistance of Klebsiella pneumoniae causing blood stream infections
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Abstract
Summary
Objective: To evaluate the value of resistant genotype to predict carbapenem resistance phenotype of K. pneumoniae causing blood stream infections. Subject and method: A total of 50 K. pneumoniae isolated from patients with BSIs, who were treated at the 108 Military Central Hospital (108 MCH), in Hanoi, from Oct. 2014 to May 2016. All strains were identified and measured antibiotic susceptibility by using Vitek II Automated system (BioMerieux, France). Genes producing beta-lactamase were screened using in-house Multiplex PCR at Vietnamese-German Center for Medical Research, 108 MCH. Result: In total, 13/50 (26%) of K. pneumoniae isolates were of carbapenem resistances. These strains carried out cabapenemase genes including NDM-1 (24%), VIM (12%), KPC (2%) and OXA-48 (2%), respectively. Frequency of NDM-1, TSC, 2POS, CN and NoC genes in carbapenem resistant strains were significantly higher than those in carbapenem susceptible strains (p<0.05). The sensitivity, specificity, positive predict value and negative predict value of these genotypes to predict resistant carbapenem were (46.2% to 84.6%), (62.2% to 91.9%), (42.1% to 66.7%) and (82.5% to 92%), respectively. Conclusion: Genotypes can be used to predict carbapenem susceptibility of K. pneumoniae causing BSIs.
Keywords: Sepsis, carbapenem resistance, K. pneumoniae, genotypic resistance.
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References
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