ISSN: 1859 - 2872
Evaluation of the efficacy of cold atmospheric plasma in promoting wound healing in an experimental diabetic rabbit model
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Abstract
To evaluate the therapeutic efficacy of cold atmospheric plasma (CAP) in accelerating wound healing in an experimental diabetic rabbit model. Subject and method: Sixteen healthy adult male New Zealand White rabbits were included. Diabetes mellitus was induced by intravenous injection of alloxan monohydrate (100mg/kg). On day 30 after confirmation of stable hyperglycemia, six circular full-thickness excisional wounds (2.5 cm in diameter) were created symmetrically across the dorsal midline. Three wounds on the left side served as controls and received standard daily dressing with 0.9% normal saline. The three wounds on the right side received the same dressing regimen plus daily CAP irradiation at 30s/cm². Clinical wound scores, percentage wound size reduction (assessed on days N1, N4, N7, N11, N14, N18, and N21), histopathological findings (days 7 and 14), and time to complete healing were compared between groups. Result: The CAP-treated group demonstrated significantly higher clinical wound scores from day N4 to N14 and greater wound contraction rates from day N4 to N11 compared with the control group (p<0.001). The mean time to complete healing was significantly shorter in the CAP group (16.75 days) than in the control group (20.31days, p<0.05). Histopathological examination on days 7 and 14 revealed significantly better re-epithelialization and granulation tissue formation in the CAP-treated wounds (p<0.05). Conclusion: Cold atmospheric plasma significantly accelerates diabetic wound healing, with the most prominent effects observed during the inflammatory and proliferative phases.
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References
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