ISSN: 1859 - 2872
Next-generation sequencing for detection of gene variants in non-small cell lung cancer tissue samples
Main Article Content
Keywords
Abstract
In next-generation sequencing (NGS) to detect genetic variants in non-small cell lung cancer (NSCLC) for targeted therapy, it is essential to obtain and validate the results of NGS to ensure the accuracy of the results and prevent false positives or negatives that could impact treatment decisions. We performed NGS to identify genetic variants for targeted therapy on 30 tissue samples from fine needle aspiration and surgical biopsies of NSCLC patients at 103 Military Hospital, utilizing the Ion Torrent system and a DNA and RNA target sequencing kit to assess variants in 81 genes. Validation through Digital PCR and Minisequencing on 30% of the samples showed 100% concordance with NGS results. This study employed a reliable approach to detect genetic variants in NSCLC, laying the foundation for the application of targeted therapy in Vietnam.
Article Details
References
2. Mina SA, Shanshal M, Leventakos K et al (2025) Emerging Targeted Therapies in Non-Small-Cell Lung Cancer (NSCLC). Cancers 17(3): 353.
3. Melosky B, Juergens RA, Banerji S et al (2025) The continually evolving landscape of novel therapies in oncogene-driven advanced non-small-cell lung cancer. Therapeutic Advances in Medical Oncology, 17, 17588359241308784.
4. Williams C, Pontén F, Moberg C et al (1999) A high frequency of sequence alterations is due to formalin fixation of archival specimens. The American journal of pathology 155(5): 1467-1471.
5. Kim S, Park C, Ji Y, Kim DG et al (2017) Deamination effects in formalin-fixed, paraffin-embedded tissue samples in the era of precision medicine. The Journal of Molecular Diagnostics 19(1): 137-146.
6. Gao XH, Li J, Gong HF et al (2020) Comparison of fresh frozen tissue with formalin-fixed paraffin-embedded tissue for mutation analysis using a multi-gene panel in patients with colorectal cancer. Frontiers in oncology 10: 310.
7. Kerick M, Isau M, Timmermann B et al (2011) Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity. BMC medical genomics 4: 1-13.
8. Owen D, Ben-Shachar R, Feliciano J et al (2024). Actionable Structural Variant Detection via RNA-NGS and DNA-NGS in Patients With Advanced Non-Small Cell Lung Cancer. JAMA network open 7(11): e2442970-e2442970.
9. Pisapia P, Iaccarino A, De Luca C et al (2024) RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: Data from Campania, Italy. Pathologica-Journal of the Italian Society of Anatomic Pathology and Diagnostic Cytopathology, 116.
Article Sidebar
