ISSN: 1859 - 2872
Hematopoietic stem cell enrichment in graft engineering by CD34+ cell selection on CliniMACS system
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Abstract
Objective: Hematopoietic stem cell enrichment by selecting CD34+ cells from mononuclear cells (MNC) collected using an apheresis machine after mobilization therapy. Subjects and methods: Four hematopoietic stem cell (HSC) harvest apheresis units were collected from four patients who were candidates for hematopoietic stem cell transplantation (HSCT). Enrichment of HSC by using microbeads directly conjugated to CD34 antibodies for magnetic labeling of CD34-expressing cells from the harvest apheresis on the CliniMACS Plus system. Results: The recovery efficiency of CD34+cell was 72.17%, and the removal efficiency of T lymphocytes, B lymphocytes, and NK cells was 99.9%. Conclusion: Immunomagnetic CD34+ cell selection CD34+ provides a promising solution to optimize HSCT by CD34+ cell enrichment in harvest apheresis
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References
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