ISSN: 1859 - 2872
Isolation, cultivation, evaluation, and storage of mesenchymal stem cells from canine adipose tissue for experimental medical research
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Abstract
Objective: Production of mesenchymal stem cells from animal (canine) adipose tissue for experimental medical research. Subject and method: Mesenchymal stem cells (MSCs) were isolated from adipose tissue of animals (canine) at different locations of the body, such as subcutaneous and visceral (mesenteric, perirenal, and ovarian) surrounding fat, and were evaluated for morphological characteristics, differentiation ability, expression level of some specific RNAs and stability of genetic factors through the culture stages. Result and conclusion: Mesenchymal stem cells isolated and cultured from subcutaneous adipose tissue can proliferate, differentiate, and express RNA of specific surface markers and maintain genetic stability through the culture stages.
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References
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