Nghiên cứu sự thay đổi các chỉ số hồng cầu của khối hồng cầu đông lạnh trước và sau deglycerol

Abstract

Objective: To evaluate changes in red blood cell indicators in frozen red cell package (FRCP) at before and immediate after deglycerolization (T₀), 7 days post-deglycerolization (T7), 14 days post-deglycerolization (T₁₄). Subject and method: We conducted a prospective, cross-sectional study on 32 frozen red blood cell units which were cryopreserved with high glycerol concentration (40%) at temperature of below -65ᵒC from December of 2017 and were deglycerolizde from June to August of 2019 at Hematology and Blood Transfusion Center, 103 Military Hospital. Result: The mean of RBC, that was 4.711 ± 0.425T/l in whole blood cell, was 4.727 ± 1.101T/l in FRCP, were 6.248 ± 0.425T/l, 6.156 ± 0.412T/l, 6.063 ± 0.397T/l, at T₀, T₇, T₁₄ respectively. The mean of HGB, that was 141.59 ± 10.12g/l in whole blood, was 140.78 ± 32.29g/l in FRCP, were 182.69 ± 11.49g/l, 179.47 ± 11.07g/l and 176.69 ± 10.71g/l at T₀, T₇ and T₁₄ respectively. The mean of HCT, that was 0.408 ± 0.025l/l in whole blood, was 0.557 ± 0.099l/l in FRCP, were 0.564 ± 0.032l/l, 0.556 ± 0.031l/l and 0.549 ± 0.030l/l at T₀, T₇ and T₁₄ respectively. MCV, that was 90.4 ± 2.02fl in whole blood, was 119.2 ± 7.6fl in FRCP, were 90.1 ± 2.3fl, 90.8 ± 2.4fl and 91.3 ± 1.8fl at T₀, T₇, T₁₄ respectively. MCH was 29.9 ± 1.3pg in whole blood and remained stability in all stages. The fluctuation of RDW was similar to MCV. Conclusion: The change in RBC, HGB, HCT post deglycerolization of FRCP produced and cryoperserved at 103 Military Hospital was similar to liquid red blood cell after deglycerolization. MCV and RDW were recognised an increase during the storage time at 2 - 6ᵒC, while the MCH was stable in all stages.