Research on developing a surgical procedure for harvest, washing and preservation of the uterus in experimental uterine transplantation in dogs

  • Nguyen Thanh Ha 108 Military Central Hospital

Main Article Content

Keywords

Uterine transplant, uterine transplant experiment on dogs

Abstract

Objective: To develop a surgical procedure to harvest, washing and preserve the uterus in experimental dogs. Subject and method: A prospective interventional study, conducted on 10 pairs of dogs at 108 Military Central Hospital from January 2020 to October 2020. Result: The uteruses of all 10 donor dogs, after being perfusion and preserved, met the standards for transplantation into the recipient dog. The average time from the start of the surgery to the time the uterus was removed from the donor dog's abdomen for 10 transplant pairs was: 70 ± 8.16 (minutes). Warm ischemia time was 34.4 ± 4.14. Conclusion: Developing a surgical procedure to harvest, washing and preserve the uterus in experimental dogs is the basis for moving forward with uterine transplantation in humans in Vietnam. These are the first cases of experimental uterus transplants on dogs performed in Vietnam.

Article Details

References

1. Eraslan S, Hamernik RJ, Hardy JD (1966) Replantation of uterus and ovaries in dogs, with successful pregnancy. Archives of surgery (Chicago, Ill: 1960) 92(1): 9-12.
2. Racho El-Akouri R, Kurlberg G, Dindelegan G, Mölne J, Wallin A, Brännström M (2002) Heterotopic uterine transplantation by vascular anastomosis in the mouse. The Journal of endocrinology 174(2): 157-166.
3. Wranning CA, Akhi SN, Kurlberg G, Brännström M (2008) Uterus transplantation in the rat: Model development, surgical learning and morphological evaluation of healing. Acta Obstet Gynecol Scand 87(11): 1239-1247.
4. Dahm-Kähler P, Wranning C, Lundmark C, Enskog A, Mölne J, Marcickiewicz J et al (2008) Transplantation of the uterus in sheep: methodology and early reperfusion events. The journal of obstetrics and gynaecology research 34(5): 784-793.