Kết quả ghép giác mạc nội mô có bóc màng Descemet thực nghiệm trên mắt chó

Abstract

Introduction: Descemet stripping endothelial keratoplasty (DSEK and DSAEK) has been commonly applied but there is still a lack of skill training models for surgeons on animals (pre-clinical training). Objective: To develope a technical process for DSEK transplantation on dog eyes suitable for training skills for corneal transplant (DSEK & DSAEK) surgeons. Subject and method: Prospective, clinical trial on 10 pairs of healthy dogs, DSEK surgery with similar techniques on human eyes at 108 Military Central Hospital during December 2021 to June 2022. Evaluate the results of graft collection technique, graft surgery, and eye condition after surgery, including central corneal thickness and graft thickness measurement by OCT. Result: Successful graft collection in 10/10 donor dog eyes; DSEK surgery was successful in 8/10 eyes with endotracheal anesthesia and good intraocular pressure control. Intra-operative difficulty was in 2 eyes with intravenous anesthesia and poor intraocular pressure control, of which 1 case failed right from surgery. Postoperative results were quite good in eyes with successful surgery. After 1 month, central corneal thickness was 675.57 ± 324.13µm (403-1270µm); graft thichness was 275 ± 109µm (127-394µm), corneal grafts was very good in 5/10 eyes. The complications were mild exudation, hemorrhage, and corneal neovascularization. Conclusion: DSEK surgery performed on living dog eyes is similar to surgery on human eyes and can be performed conveniently with endotracheal anesthesia and appropriate intraocular pressure adjustment. Training skills for surgeons to DSEK on a dog eye model is appropriate.