ISSN: 1859 - 2872
Designing in silico primers and probes for the real-time PCR detection and serotype identification of dengue virus in Vietnam
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Abstract
Objective: To design in silico primer and probe sets for the development of a multiplex real-time PCR diagnostic and serotyping kit for the dengue virus, utilizing mostly genomic data isolated from Vietnamese patients. Subject and method: A total of 1,718 complete genome sequences of dengue virus strains from the database (covering the years 2001 to 2022) were used, including 639 (37.2%) complete genome sequences of dengue virus strains collected in Vietnam. Using bioinformatics tools, we designed in silico primers and probes sets for multiplex real-time PCR diagnosis and serotyping of the dengue virus in Vietnam. Result and conclusion: The results obtained include four pairs of primers and probes, with target gene sizes of 106bp, 98bp, 156bp, and 103bp, which are specific to dengue virus serotypes 1, 2, 3, and 4, respectively.
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References
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