ISSN: 1859 - 2872
Evaluating the efficacy of allogeneic adipose-derived mesenchymal stem cells in hyaline cartilage regeneration in a dog model
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Abstract
Objective: Cartilage damage is frequently recognized as a source of knee arthritis, which can cause many long-term complications. Among existing cartilage treatment options, mesenchymal stem cells (MSCs) have emerged as promising treatments for cartilage regeneration. This study aimed to comprehensively evaluate the cartilage regeneration ability of adipose-derived allogeneic AD-MSCs for acute cartilage defects in an experimental model. Subject and method: This model is based on surgically applied mechanical articular cartilage damage of all four limb joints of sixteen dogs selected for the study. They were symmetrical randomized to a single infusion either of placebo or (2´106 cells) allogeneic AD-MSCs and followed for 3 months. The effectiveness of treatment was validated by gross anatomy scores and histopathology scores. Result: Higher macroscopic were observed in MSCs-treated joints throughout the study period, with significant differences noted at 3 months (9.5 ± 0.6 vs 4.3 ± 1.5, p<0.05) and 6 months (8.7 ± 1.2 vs 4.6 ± 1.7, p<0.05), respectively. There was a significantly higher amount of hyaline cartilage in the MSC treatment group than in the placebo group. No adverse event was observed during the 3-month follow-up after treatment. Conclusion: Allogeneic AD-MSCs showed efficiency in producing hyaline cartilage, which might be more advanced than the current surgical techniques that deliver only fibrocartilage. The results suggest a promising application of MSC treatment for articular cartilage defects
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References
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