ISSN: 1859 - 2872
Performance evaluation of multiplex real-time PCR for dengue virus detection using in-house primer and probe designed based on dengue virus sequence isolated from Vietnam
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Abstract
Objective: The study aimed to evaluate a multiplex real-time PCR for serotyping dengue virus using primers and probe designed by the research team, using genetic data from dengue viruses isolated from patients in Vietnam. Subject and method: A total of 284 blood sample specimens from patients diagnosed with dengue fever were collected at the 108 Military Central Hospital during the period from October 2021 to December 2022, in Hanoi. RNA of Dengue virus were extracted and used to assess the in-house developped kit, which was compared with the CDC (U.S. Centers for Disease Control and Prevention) diagnostic kit (CE-IVD). Result: The in-house developped kit procedure had a detection threshold of 100 copies/μL and a specificity of 100%. While performing on 284 samples, the in-house developped kit exhibited a slightly higher sensitivity for dengue virus detection compared to the CDC kit (71.5% positive and 63.0% positive, respectively). Conclusion: Thus, the in-house developped kit demonstrates high technical specificity, with sensitivity on diagnostic specimens for dengue fever comparable to the kit issued by the CDC US.
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References
2. Dwivedi VD, Tripathi IP, Tripathi RC, Bharadwaj S, Mishra SK (2017) Genomics, proteomics and evolution of dengue virus. Brief Funct Genomics 16(4): 217-227.
3. Hung TM, Clapham HE, Bettis AA et al (2018) The Estimates of the Health and Economic Burden of Dengue in Vietnam. Trends Parasitol 34(10): 904-918. doi:10.1016/j.pt.2018.07.007.
4. Phadungsombat J, Vu HTT, Nguyen QT, Nguyen HTV, Nguyen HTN, Dang BT, Nakayama EE, Ishizaki A, Ichimura H, Shioda T, Pham TN (2023) Molecular Characterization of Dengue Virus Strains from the 2019–2020 Epidemic in Hanoi, Vietnam. Microorganisms. 11(5):1267. doi: 10.3390/microorganisms11051267.
5. Vicente CR, Herbinger KH, Fröschl G, Romano CM, Cabidelle A de SA, Junior CC (2016) Serotype influences on dengue severity: A cross-sectional study on 485 confirmed dengue cases in Vitória, Brazil. BMC Infect Dis 16(1): 1-7.
6. Rocha BAM, Guilarde AO, Argolo AFLT et al (2017) Dengue-specific serotype related to clinical severity during the 2012/2013 epidemic in centre of Brazil. Infect Dis poverty 6(04): 73-83.
7. Centre for Disease Control and Prevention (2013) CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus The CDC Real Time RT-PCR Assay for Dengue Diagnosis. Cdc. Published online:1-5.
8. Vuong NL, Ha Quyen NT, Hanh Tien NT et al (2024) Dengue viremia kinetics and effects on platelet count and clinical outcomes: An analysis of 2340 patients from Vietnam. Published online. doi:10.7554/elife.92606.2.
9. Nabeshima T, Ngwe Tun MM, Thuy NTT et al (2023) An outbreak of a novel lineage of dengue virus 2 in Vietnam in 2022. J Med Virol 95(11): 29255.
10. Nguyễn Đức Thuận, Đặng Thành Chung (2021) Nghiên cứu Tỉ lệ type virus dengue ở bệnh nhân nhi trong một số đợt dịch tại khu vực miền Nam Việt Nam. Tạp chí Y học Việt Nam 503(1). doi:10.51298/vmj.v503i1.695.
11. Senaratne UTN, Senaratne UTN, Murugananthan K et al (2020) Dengue virus co-infections with multiple serotypes do not result in a different clinical outcome compared to mono-infections. Epidemiol Infect. Published online :0-8. doi:10.1017/S0950268820000229.
12. Dhanoa A, Hassan SS, Ngim CF et al (2016) Impact of dengue virus (DENV) co-infection on clinical manifestations, disease severity and laboratory parameters. BMC Infect Dis 16: 1-14.
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