ISSN: 1859 - 2872
Performance evaluation of multiplex real-time PCR for dengue virus detection using in-house primer and probe designed based on dengue virus sequence isolated from Vietnam
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Abstract
Objective: The study aimed to evaluate a multiplex real-time PCR for serotyping dengue virus using primers and probe designed by the research team, using genetic data from dengue viruses isolated from patients in Vietnam. Subject and method: A total of 284 blood sample specimens from patients diagnosed with dengue fever were collected at the 108 Military Central Hospital during the period from October 2021 to December 2022, in Hanoi. RNA of Dengue virus were extracted and used to assess the in-house developped kit, which was compared with the CDC (U.S. Centers for Disease Control and Prevention) diagnostic kit (CE-IVD). Result: The in-house developped kit procedure had a detection threshold of 100 copies/μL and a specificity of 100%. While performing on 284 samples, the in-house developped kit exhibited a slightly higher sensitivity for dengue virus detection compared to the CDC kit (71.5% positive and 63.0% positive, respectively). Conclusion: Thus, the in-house developped kit demonstrates high technical specificity, with sensitivity on diagnostic specimens for dengue fever comparable to the kit issued by the CDC US.
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References
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