ISSN: 1859 - 2872
Study on changes in WBC, K+ concentration and pH level in frozen red blood cell package before and after deglycerolization
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Abstract
Objective: To evaluate the change in white blood cell, potassium concentration and pH level in frozen red cell package (FRCP) at before deglycerolized, immediate time after deglycerolized (T0), 7 days post-deglycerolized (T7), 14 days post-deglycerolized (T14). Subject and method: We conducted a prospective, cross-sectional study on 32 frozen red blood cell units which were cryopreserved with high glycerol concentration (40%) at temperature of below -65oC from December of 2017 and were deglycerolized from June to August of 2019 at Hematology and Blood Transfusion Center, 103 Military Hospital. Result: WBC, that was 3.47 ± 1.33G/l in FRCP, were 0.060 ± 0.019G/l, 0.058 ± 0.022G/l and 0.055 ± 0.024G/l at T0, T7 and T14 respectively. Deglycerolization omitted 98.68% of WBC unchanged during storage time after deglycerolization. Average of K+ concentration level, that was 4.94 ± 0.76mmol/l at before deglycerolized, were 1.21 ± 0.39mmol/l, 11.73 ± 2.41mmol/l and 17.55 ± 3.00mmol/l, at T0, T7 and T14 respectively. K+ concentration increased significantly stastically during storage time after deglycerolization (p<0.01), T0, T7 and T14 pH level were 6.65 ± 0.027, 6.55 ± 0.044 and 6.47 ± 0.046, respectively. pH statistically decreased during storage time after deglycerol. Conclusion: Deglycerolization omitted almost of WBC in frozen red blood cell package. pH level decreased steadily and K+ concentration increased gradually during storage time after deglycerolization.
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References
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