The variation of the microbiology in endodontic treatment of periapical periodontitis using new generation sequenced 16S rDNA metagenomics

  • Dương Thị Phương Linh Bệnh viện Răng Hàm Mặt Trung ương Hà Nội
  • Nguyễn Vũ Trung Viện Pasteur TP. Hồ Chí Minh
  • Nguyễn Hữu An Viện Công nghệ Phacogen

Main Article Content

Keywords

Metagenomics, root canal irrigation

Abstract

Objective: To describe the composition and fluctuations of the microbiota in root cannal of teeth with periapical periodontitis through stages of endodontic treatment. Subject and method: Longitudinal study on 32 teeth of patients examined at Hanoi National Hospital of Odonto-Stomatology from April 2020 to April 2021. Patients have teeth with periapical periodontitis were divided into 2 groups: Group I were irrigated into root canal by chloherxidine (CHX) 2% combined with sodium hypochlorite (NaOCl) 3%; group II were irrigated into root canal by 3% NaOCl. Take samples in root cannal in 3 times: Before irrigation (S1), after irrigation (S2), and 2 weeks after place the medicine in root canal (S3). Result: There were 27 phyla and 467 genera of microorganisms in 96 samples. The dominant microbial genera tended to gradually decrease in 2 groups, in which group I had a higher level of reduction of these dominant genera than in group II. Conclusion: Using NaOCl disinfectant solution combined with CHX can eliminate microorganisms on a large scale, and change the proportion of microorganisms in the population more than NaOCl.

Article Details

References

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Published
Jul 24, 2024
DOI: https://doi.org/10.52389/ydls.v19i4.2248
Issue
Volume 19 - No 4/2024
Section
Articles
How to Cite
LinhD. T. P., TrungN. V., & AnN. H. (2024). The variation of the microbiology in endodontic treatment of periapical periodontitis using new generation sequenced 16S rDNA metagenomics. Tạp Chí Y Dược lâm sàng 108, 19(4). https://doi.org/10.52389/ydls.v19i4.2248