ISSN: 1859 - 2872
The initial assessment of microRNA-21 expression levels in peripheral blood in gastric cancer in Vietnamese patients
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Abstract
Objective: This study aimed to refine the evaluation process of miR-21 expression levels in the peripheral blood of Vietnamese individuals and to preliminarily assess its value in supporting the diagnosis of gastric cancer (GC). Subject and method: In 2023, 35 GC patients (Patient group) and 35 non-GC individuals (Control group) were examined and treated at the 108 Military Central Hospital. MiR-21 circulating freely in peripheral blood was detected in patients using a real-time PCR technique employing a specific stem-loop primer set; expression levels were assessed using the Livak formula. The diagnostic support value of miR-21 for GC was evaluated through the area under the receiver operating characteristic curve (AUC) and sensitivity-specificity (compared to CEA, CA19-9, and CA72-4). Result: The expression level of miR-21 was determined using a qPCR technique employing the miR-internal standard gene. The results indicated that miR-21 expression in the SC group was twice as high as in the control group. Moreover, miR-21 exhibited better diagnostic support for SC compared to CEA, CA19-9, and CA72-4, as it had the highest AUC (0.64 vs. 0.48 vs. 0.57 vs. 0.47, respectively). Conclusion: MicroRNA-21 expression is elevated in Vietnamese patients with GC, and it may be utilized as a potential biomarker to diagnose the disease. However, further in-depth and extensive studies are warranted.
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References
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