Characteristics of gut microbiota at colorectal cancer tissue, metastatic liver and lymph nodes tissue in colorectal cancer patients

  • Nguyễn Duy Trường Bệnh viện Trung ương Quân đội 108
  • Ngô Tất Trung Bệnh viện Trung ương Quân đội 108
  • Trần Thị Thanh Huyền Bệnh viện Trung ương Quân đội 108
  • Đào Thanh Quyên Bệnh viện Trung ương Quân đội 108
  • Đào Thị Huyền Bệnh viện Trung ương Quân đội 108
  • Phạm Quang Trung Bệnh viện Trung ương Quân đội 108
  • Lê Hữu Song Bệnh viện Trung ương Quân đội 108

Main Article Content

Keywords

16S rRNA metagenomic, Colorectal cancer

Abstract

Objective: To describe gut microbiota composition and diversity at colorectal cancer (CRC) tissue, metastatic liver and lymph nodes tissue in CRC patients. Subject and method: 21 patients with CRC at stage IV treated at 108 Military Central Hospital from March 2015 to August 2018 were recruited into the current cross-sectional study. Clinical information and tissue samples (include 21 CRC tissue samples, 21 metastatic liver tissue samples and 11 metastatic lymph nodes tissue samples) were collected from the CRC patients. Sequencing of the 16S rRNA gene targeted V3-V4 region was used to access gut microbiota composition and diversity of the studied samples. Result: The phyla accounting for the highest proportion in the three types of tissue samples included Proteobacteria, Actinobacteriota, Firmicutes, Bacteroidota, Deinococcota, and Fusobacteriota. The alpha diversity in the CRC tissue samples was higher than in the  metastatic liver tissue samples (p<0.05). There was significant difference in beta diversity between the CRC tissue and the metastatic liver, and between the CRC tissue and the metastatic lymph nodes (p<0.05). Conclusion: Preliminary analysis determined the characteristics of gut microbiota at CRC tissue, metastatic liver and lymph nodes tissue using 16S rRNA metagenomics.

Article Details

References

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Published
Dec 29, 2023
DOI: https://doi.org/10.52389/ydls.v18i8.2096
Issue
Volume 18-No 8/2023
Section
Articles
How to Cite
TrườngN. D., TrungN. T., HuyềnT. T. T., Quyên Đào T., Huyền Đào T., TrungP. Q., & SongL. H. (2023). Characteristics of gut microbiota at colorectal cancer tissue, metastatic liver and lymph nodes tissue in colorectal cancer patients. Tạp Chí Y Dược lâm sàng 108, 18(8). https://doi.org/10.52389/ydls.v18i8.2096