ISSN: 1859 - 2872
Preliminary evaluation of gut microbiota diversity in colorectal cancer patients using 16S rRNA sequencing
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Abstract
Objective: To study gut microbiota composition and diversity in colorectal cancer patients (CRCs) compared to healthy controls by 16S rRNA sequencing in feacal samples. Subject and method: Clinical information and feacal samples from 10 newly diagnosed CRCs and 05 healthy control were collected at 108 Military Central Hospital. Total genomic DNA were extracted from feacal samples and subjected to 16S rRNA V3-V4 region sequencing. Bioinformatics and statistical analysis allowed to compare the microbiota composition and diversity between CRC patients and healthy controls. Result: Total DNA of 15 feacal samples was extracted successfully. There was no significant difference in alpha and beta diversity between the two groups. The composition of bacterial species belonging to 25 genera, 16 families, and 5 phyla accounted for a high proportion in CRC and healthy samples. The relative abundance of bacterial taxa varied between the two studied groups and also between participants within the same group. Conclusion: Initially using 16S rRNA gene sequencing technique, we have successfully determined the composition of gut bacteria to genus. This pilot study works as a good foundation for further analysis that use a larger sample size to increase statistical power and to allow for differences between CRC patients and healthy controls to be detected.
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References
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