Nghiên cứu điều chế và kiểm tra chất lượng 166Ho-DOTMP - tác nhân tiềm năng điều trị giảm đau do ung thư di căn xương

Abstract

Objective: ¹⁶⁶Ho-DOTMP are radiolabeled molecules which are used to deliver sufficient doses of ionizing radiation to bone marrow in the therapy of bone metastases and bone marrow malignancies. In this paper, the production and quality control of ¹⁶⁶Ho-DOTMP have been presented. Subject and method: DOTMP was labeled with ¹⁶⁶Ho by direct radiolabeled method which which created coordination bonds with the Ho³⁺ ion in a neutral medium. The optimization conditions of radiolabeling procedure such as pH, time, temperature and reactants molar ratio was carried out. Thereafter, radiolabeling yield, radiochemical purity and stability of ¹⁶⁶Ho-DOTMP complexes were investigated using paper chromatography. Result: The results show that the radiolabeling efficiency in the preparation of ¹⁶⁶Ho-DOTMP was more than 98% with the reactants molar ratio of 1:30, 1:40 and 1:50.  The radioactivity of ¹⁶⁶Ho was measured about 10-40mCi. The reaction conditions were gained at the pH 6-8 for 15 minutes and under the condition of the 25⁰C temperature. The radiolabeling yields and radiochemical purity were aboved 98% and stability for 3 days storage. Conclusion: The ¹⁶⁶Ho-DOTMP complexes initially reach the required criteria of radiopharmaceutical for continuing preclinical evaluations.