Tetra-primer ARMS PCR optimization to detect single nucleotide polymorphism rs2062345 of SOCS6 gene in patients with hepatitis B virus

  • Nguyễn Việt Phương Bệnh viện Quân y 103
  • Lê Văn Khánh Viện Nghiên cứu Y dược học Quân sự
  • Hoàng Văn Tổng Viện Nghiên cứu Y dược học Quân sự
  • Nguyễn Thanh Tuấn Bệnh viện Trung ương Quân đội 108
  • Vũ Duy Minh Bệnh viện Trung ương Quân đội 108
  • Trần Viết Tiến Bệnh viện Quân y 103

Main Article Content

Keywords

Single nucleotide polymorphism, suppressor of cytokine signaling 6 gene, Tetra-primer amplification refractory mutation system-polymerase chain

Abstract

Objective: To construct a protocol to detect single nucleotide polymorphism (SNP) rs2062345 of SOS6 gene in patients with hepatitis B virus using Tetra-primer ARMS PCR (T-ARMS PCR). Subject and method: Venous blood samples from patients with hepatitis B virus (chronic hepatitis B, cirrhosis, hepatocellular carcinoma), using the T-ARMS PCR method; compared some representative samples with Sanger gene sequencing method. Result: The T-ARMS PCR technique has been optimized to detect the SNP rs2062345 of SOCS6 gene. The T-ARMS PCR technique was designed and optimized to detect the SNP rs2062345 of SOCS6 gene. The PCR electrophoresis results were clearly visible, there were no extra band and matched the genotype of SNP rs2062345 of they SOCS6 gene, completely similar to the Sanger sequencing method. Conclusion: T-ARMS PCR has been designed, and optimized to identify SNP rs2062345 of SOCS6 gene in patients with hepatitis B virus.

Article Details

References

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Published
Aug 7, 2023
DOI: https://doi.org/10.52389/ydls.v18i5.1901
Issue
Volume 18-No 5/2023
Section
Articles
How to Cite
PhươngN. V., KhánhL. V., TổngH. V., TuấnN. T., MinhV. D., & TiếnT. V. (2023). Tetra-primer ARMS PCR optimization to detect single nucleotide polymorphism rs2062345 of SOCS6 gene in patients with hepatitis B virus. Tạp Chí Y Dược lâm sàng 108, 18(5). https://doi.org/10.52389/ydls.v18i5.1901