ISSN: 1859 - 2872
Investigating the role of cagPAI of Helicobacter pylori in gastric cancer patients
Main Article Content
Keywords
Abstract
Objective: To investigate the relationship between H. pylori’s cagPAI intactness and the risk of gastric cancer (GC) in Vietnam. Subject and method: A cross-sectional study of 179 patients with H. pylori infection, in which 89 patients with gastric cancer and 90 patients with chronic gastritis (CG). Diagnosis of GC and CG is based on histopathology. CagPAI status was determined through the present or absent of three genes cagA, cagE, cagT by realtime PCR technique. Result: 100% of H. pylori strains carried cagPAI gene, in which the rate of H. pylori carrying the intact cagPAI was 70.4%. The GC group had a higher rate of intact cagPAI than the CG group (79.8% vs 61.1%), the difference was statistically significant (OR = 2.5, p<0.01). In gastric cancer patients: There was no difference in the prevalence of H. pylori carrying the cagPAI gene by age group, gender, macroscopic characteristics on endoscopy (according to Borrmann classification) and on histopathology (according to the Lauren's classification). Conclusion: The intact cagPAI of H. pylori is associated with the risk of gastric cancer in Vietnam.
Article Details
References
2. Kusters JG, van Vliet AH, Kuipers EJ (2006) Pathogenesis of Helicobacter pylori infection. Clinical microbiology reviews 19(3): 449-490.
3. Fischer W, Püls J, Buhrdorf R, Gebert B, Odenbreit S, Haas R (2001) Systematic mutagenesis of the Helicobacter pylori cag pathogenicity island: Essential genes for CagA translocation in host cells and induction of interleukin‐8. Molecular microbiology 42(5): 1337-1348.
4. Ikenoue T, Maeda S, Ogura K et al (2001) Determination of Helicobacter pylori virulence by simple gene analysis of the cag pathogenicity island. Clin Diagn Lab Immunol 8(1): 181-186.
5. Khatoon J, KN Prasad, R Prakash Rai et al (2017) Association of heterogenicity of Helicobacter pylori cag pathogenicity island with peptic ulcer diseases and gastric cancer. Br J Biomed Sci 74(3): 121-126.
6. Day AS, Jones NL, Lynett JT, Jennings HA, Fallone CA, Beech R, Sherman PM (2000) cagE Is a virulence factor associated with helicobacter pylori induced duodenal ulceration in children. The Journal of infectious diseases 181(4): 1370-1375.
7. Mattar R, Marques SB, Monteiro MDS, Dos Santos AF, Iriya K, Carrilho FJ (2007) Helicobacter pylori cag pathogenicity island genes: Clinical relevance for peptic ulcer disease development in Brazil. Journal of medical microbiology 56(1): 9-14.
8. Nguyen LT, Uchida T, Tsukamoto Y et al (2010) Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam. European journal of clinical microbiology infectious diseases 29(6): 651-660.
9. Nilsson C, Sillén A, Eriksson L et al (2003) Correlation between cag pathogenicity island composition and Helicobacter pylori-associated gastroduodenal disease. Infect Immun 71(11): 6573-6581.
10. Amieva M and Peek RM (2016) Pathobiology of Helicobacter pylori induced gastric cancer. Gastroenterology 150(1): 64-78.
Article Sidebar
