ISSN: 1859 - 2872
Application of AMROMICS bioinformatics tool to automatic analysis of bacterial whole genome sequencing data
Main Article Content
Keywords
Abstract
Objective: To investigate initially the application of the AMROMICS bioinformatics tool in the automatic analysis of bacterial genomes. Subject and method: Whole-genome analysis of 14 E. coli isolates and the standard E. coli K-12 MG1655 strain published on NCBI database using the AMROMICS bioinformatics tool. Result: AMROMICS automatically analyzed the entire genome of 15 bacterial samples in a short time, and simultaneously showed genome structure, sequence type, virulence gene, and resistance gene with a simple and easy-to-use interface. Most strains could be multi-drug resistant bacteria and harbor genes associated with resistance to commonly used antibiotics such as cephalosporin, quinolone and aminoglycoside. Conclusion: The AMROMICS tool has the potential for clinical application, with the ability to quickly determine drug-resistant genes and virulence genes, AMROMICS can be applied in developing effective treatments regimens and infection control measures.
Article Details
References
2. Nguyễn Thị Tuyền, Lê Nữ Xuân Thanh & Ngô Viết Quỳnh Trâm (2021) Tình hình kháng kháng sinh của các chủng E. Coli và đặc điểm gen mã hóa carbapenemase của các chủng E. coli kháng carbapenem phân lập được tại Bệnh viện Trường Đại học Y - Dược Huế. Tạp chí Y dược học 11.
3. Bộ Y tế, Việt Nam xuất hiện siêu vi khuẩn kháng tất cả kháng sinh, bác sĩ bất lực. Available at: https://moh.gov.vn/chuong-trinh-muc-tieu-quoc-gia/-/asset_publisher/7ng11fEWgASC/content/ viet-nam-xuat-hien-sieu-vi-khuan-khang-tat-ca-khang-sinh-bac-si-bat-luc. (Truy cập: 3rd June 2022)
4. Varughese LR et al (2018) Analytical profiling of mutations in quinolone resistance determining region of gyrA gene among UPEC. PLoS One 13.
5. Blot M, Meyer J & Arber W (1991) Bleomycin-resistance gene derived from the transposon Tn5 confers selective advantage to Escherichia coli K-12. Proc. Natl. Acad. Sci. U. S. A. 88: 9112.
6. Blot M, Hauer B & Monnet G (1994) The Tn5 bleomycin resistance gene confers improved survival and growth advantage on Escherichia coli. Mol. Gen. Genet. 242: 595-601.
Article Sidebar
