ISSN: 1859 - 2872
Effects of p16 and RASSF1A on hepatocellular carcinoma patients
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Abstract
Epigenetic changes in gene expression due to CpG island methylation are the main cause of p16 gene inactivation. Recently, it has been suggested that the RASSF1A and p16 genes can be inactivated by methylation. Objective: To analyze the impact factors and the methylation of tissue samples in liver cancer patients. Subject and method: Testing of age groups, factors affecting hepatocellular carcinoma and the influence of methylation of two factors RASSF1A and p16 on cancer patients. Result: Hepatocellular carcinoma patients were aged from 50 to 70 years old, the highest rate 42% (p<0.05) in the age group from 60 to 70, and men had a higher incidence than women (male: 74%, female: 26%, p<0.05). The number of smokers in HCC accounted for 42.85 - 100% depending on age and the rate of people who drink alcohol in HCC was relatively low (16.67 - 19.05%) and concentrated in the age group from 50 to 70. The rate of HbsAg-positive patients was 55.56 - 100% and concentrates on 40 - 70 years old. The positive rate for anti HCV was low (28.57 - 33.33%) and concentrates in the age group from 50 to 70 years old. The percentage of samples containing p16 accounted for 44%, including methylation 63.64% in liver cancer patients and 36.36% in normal people with size of 93bp. RASSF1A samples accounted for 58% of which methylation 60.35% in liver cancer patients and 39.65% in normal people with size of 145bp. Methylation of p16 and RASSF1A in patients over 65 years of age occurs more frequently than in other age groups, and the distribution of these 2 loci in HCV-positive patients is higher than others. Conclusion: Methylation of p16 and RASSF1A is strongly associated with hepatocellular carcinoma patients and is dependent on age and sex.
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References
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