Evaluation of an automated chemiluminescence assay for detection of syphilis infection from blood donors at 108 Military Central Hospital

  • Le Huy Khoi 108 Military Central Hospital
  • Do Giang Binh 108 Military Central Hospital
  • Nguyen Xuan Thanh 108 Military Central Hospital
  • Vu Hoang Trung Thai Nguyen University of Medicine and Pharmacy
  • Pham Thi Thu Huong 108 Military Central Hospital
  • Vu Xuan Nghia 108 Military Central Hospital

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Keywords

Blood donor, syphilis, VITROS(®) syphilis TPA

Abstract

Objective: The aim of this study was to evaluate VITROS(®) syphilis Treponema pallidum agglutination (VITROS(®) syphilis TPA) assay based on enhanced chemiluminescence principle for detection of syphilis infection in blood donors. Subject and method: A total of 110 random blood samples collected from the blood donors and 30 syphilis known sero-reactive samples stored at -20°C, were used to evaluate the performance of VITROS(®) syphilis TPA assay based on enhanced chemiluminescence assay on VITROS(®) immunodiagnostics system to analyze the terms of its sensitivity, precision, cross-reactivity and interference studies. Result: The results showed that VITROS(®) syphilis TPA got 100% sensitivity and specificity with precision (20 days study) of < 10% co-efficient of variation. There was no cross-reactivity with other viral and auto-immune antibodies. No interference was observed from endogenous interfering substances like free hemoglobin or fats. Conclusion: The results suggested that VITROS(®) syphilis TPA should be done to further reduce the risk of transfusion-transmitted TP infection, decrease unnecessary blood waste and loss of blood donors.

Article Details

References

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Published
Dec 13, 2021
DOI: https://doi.org/10.52389/ydls.v16iTA.1116
Issue
Volume 16 - December 2021
Section
Articles
How to Cite
KhoiL. H., BinhD. G., ThanhN. X., TrungV. H., HuongP. T. T., & NghiaV. X. (2021). Evaluation of an automated chemiluminescence assay for detection of syphilis infection from blood donors at 108 Military Central Hospital. Tạp Chí Y Dược lâm sàng 108, 16(TA). https://doi.org/10.52389/ydls.v16iTA.1116