ISSN: 1859 - 2872
Research on the application of octacalcium phosphate/collagen composite to carry low-dose recombinant human bone morphogenetic protein-2 in bone regeneration
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Abstract
Objective: To evaluate the effective use of octacalcium phoshate/collagen (OCP/Col) as a carrier to reduce the concentration of recombinant human bone morphogenetic protein 2 (rhBMP-2) but still be effective. Subject and method: A cross-sectional descriptive experimental study with comparative control. Atelocollagen-ACS or OCP/Col sponge discs were impregnated with different concentrations of rhBMP-2 and implanted in 60 mice with calvarial bone defects. The transplanted tissue was obtained after 4, 6 weeks. Result: There was perform hard tissue formation in the defects of the OCP/Col groups at all concentrations of rhBMP-2. The ACS group had 0.25μg of rhBMP-2 showed almost no bone formation. Bone mineral density in all groups was independent of rhBMP-2 concentration. Progressive bone formation was dependent on rhBMP-2 concentration in both groups. The new bone area was significantly more in the OCP/Col groups than in the ACS groups. Conclusion: OCP/Col can be a carrier of rhBMP-2, reducing the effective dose of rhBMP-2 by up to 1/4, avoiding complications caused by rhBMP-2 at high doses.
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References
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