Evaluation of an automated chemiluminescence assay for detection of syphilis infection from blood donors at 108 Military Central Hospital

  • Le Huy Khoi 108 Military Central Hospital
  • Do Giang Binh 108 Military Central Hospital
  • Nguyen Xuan Thanh 108 Military Central Hospital
  • Vu Hoang Trung Thai Nguyen University of Medicine and Pharmacy
  • Pham Thi Thu Huong 108 Military Central Hospital
  • Vu Xuan Nghia 108 Military Central Hospital

Main Article Content

Keywords

Blood donor, syphilis, VITROS(®) syphilis TPA

Tóm tắt

Objective: The aim of this study was to evaluate VITROS(®) syphilis Treponema pallidum agglutination (VITROS(®) syphilis TPA) assay based on enhanced chemiluminescence principle for detection of syphilis infection in blood donors. Subject and method: A total of 110 random blood samples collected from the blood donors and 30 syphilis known sero-reactive samples stored at -20°C, were used to evaluate the performance of VITROS(®) syphilis TPA assay based on enhanced chemiluminescence assay on VITROS(®) immunodiagnostics system to analyze the terms of its sensitivity, precision, cross-reactivity and interference studies. Result: The results showed that VITROS(®) syphilis TPA got 100% sensitivity and specificity with precision (20 days study) of < 10% co-efficient of variation. There was no cross-reactivity with other viral and auto-immune antibodies. No interference was observed from endogenous interfering substances like free hemoglobin or fats. Conclusion: The results suggested that VITROS(®) syphilis TPA should be done to further reduce the risk of transfusion-transmitted TP infection, decrease unnecessary blood waste and loss of blood donors.

Article Details

Các tài liệu tham khảo

1. David HD and Joy HG (1990) Congenital syphilis presenting in infants after the newborn period. Engl J Med 323: 1299-1302.
2. Egglestone SI et al (2000) Serological diagnosis of syphilis communicable disease and public health. Commun Dis Public Health 3(3): 158-162.
3. Lesinski J, Krach J, Kadziewicz E (1974) Specificity, sensitivity, and diagnostic value of the TPHA test. Br J Vener Dis 50: 334-340.
4. Castro R, Prieto ES, Santo I, Azevedo J, Exposto Fda L (2003) Evaluation of an enzyme immunoassay technique for detection of antibodies against Treponema pallidum. J Clin Microbiol 41: 250-253.
5. Lefevre JC, Bertrand MA, Bauriaud R (1990) Evaluation of the Captia enzyme immunoassays for detection of immunoglobulins G and M to Treponema pallidum in syphilis. J Clin Microbiol 28: 1704-1707.
6. Young H, Pryde J, Duncan L, Dave J (2009) The architect syphilis assay for antibodies to Treponema pallidum: An automated screening assay with high sensitivity in primary syphilis. Sex Transm Infect 85: 19-23.
7. Pope V (2004) Use of treponemal tests to screen for syphilis. Infect Med 21: 399-404.
8. Reisner BS, Mann LM, Tholcken CA, Waite RT, Woods GL (1997) Use of the Treponema pallidum-specific captia syphilis IgG assay in conjunction with the rapid plasma reagin to test for syphilis. J Clin Microbiol 35: 1141-1143.
9. Knight CS, Crum MA, Hardy RW (2007) Evaluation of the LIAISON chemiluminescence immunoassay for diagnosis of syphilis. Clin Vaccine Immunol 14: 710-713.
10. VITROS® Immunodiagnostic Products Syphilis TPA Assay [Package Insert]. New Brunswick, NJ: Ortho-Clinical Diagnostics, Inc.; 2011.

Article Sidebar

PDF (English)
Đã đăng
Dec 13, 2021
DOI: https://doi.org/10.52389/ydls.v16iTA.1116
Số tạp chí
Tập 16 - Số tiếng Anh 12/2021
Chuyên mục
Articles
Trích dẫn bài viết này
KhoiL. H., BinhD. G., ThanhN. X., TrungV. H., HuongP. T. T., & NghiaV. X. (2021). Evaluation of an automated chemiluminescence assay for detection of syphilis infection from blood donors at 108 Military Central Hospital. Journal of 108 - Clinical Medicine and Phamarcy, 16(TA). https://doi.org/10.52389/ydls.v16iTA.1116