ISSN: 1859 - 2872
PDGFRα controls formation and differentiation of neurospheres in the subventricular zone of the adult mouse brain
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Objective: To evaluate the role of PDGFRa for formation and differentiation of neurospheres in the subventricular zone of the adult mouse brain. Subject and method: We used CAGG-CreER, Pdgfra flox/flox mice (CAGG-Flox mice) as control group and CAGG-CreER mice as knockout mice to inactivate PDGFRa gene via tamoxifen treatment, then examine stem cell activities in adult mouse brain by expressing formation and differentiation of neurospheres. Result: Before tamoxifen treatment the number of neurosphere and cell number of differentiation are comparable between two groups, however after tamoxifen treatment those are severely suppressed in CAGG-CreER mice. Conclusion: Formation and differentiation of neurosphere were maintained by PDGFRa gene which expressed partly stem cell activities in adult mouse brain.
Keywords: PDGFRa, neurospheres, subventricular zone, mouse brain.
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